解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Bacillus subtilis strain SO113 secretes a pectate lyase which is produced during the exponential death phase of growth, just before sporulation. This extracellular pectate lyase, which produces unsaturated products from polygalacturonate, was purified 35-fold from the culture supernatant of Bacillus subtilis by a CM S...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(90)90053-j
更新日期:1990-09-01 00:00:00
abstract::A number of specific Fab and Fv fragments and their complexes with antigens (avian lysozymes), haptens, and anti-idiotopic Fabs have been studied by immunochemical and crystallographic techniques. Antigen and antibody interact through closely complementary contacting surfaces, without major conformational changes. An ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(90)90114-v
更新日期:1990-08-01 00:00:00
abstract::Directed mutagenesis has been used to study the nicotinamide subsite of the glycolytic NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Residue Asn313 is involved together with the carboxyamide moiety of the nicotinamide ring in a complex network of hydrogen bonding interactions which fix the position o...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(90)90119-2
更新日期:1990-08-01 00:00:00
abstract::The number of proteins known to contain collagen-like triple helical domains is rapidly increasing. The functions of these domains are to provide molecular rods that separate spatially non-triple helical domains with varied properties and structures and to permit lateral interactions between molecules. Two-thirds of t...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(90)90071-n
更新日期:1990-06-01 00:00:00
abstract::Using a new extended set of 13 amber suppressors in E coli, systematic amino-acid replacements were performed at positions 104(E) and 238(G) of TEM-1 beta-lactamase from PUC19. The enzyme is tolerant to most substitutions tested at position 104. Missense revertants E104K, E104S or E104Y exhibited only minor changes in...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(90)90073-p
更新日期:1990-06-01 00:00:00
abstract::Analysis of the nucleotide sequence of 1,400 transfer RNAs has revealed the imprint of a prototypic genetic code in position 3-4-5 of the acceptor stem. It appears only in the transfer RNAs for the primordial amino acids ie those found by chemical condensation of a nitrogen-methane-water-ammonia mixture. The model for...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(90)90033-d
更新日期:1990-05-01 00:00:00
abstract::A HU-like protein (HBl) of Bifidobacterium longum was purified and characterized. HBl is heat-stable and acid-resistant, and has a molecular weight of about 9.1 kDa as estimated by its mobility on electrophoresis. HBl is intermediate in basicity (pI 9.8) between the HU-1 and HU-2 proteins of Escherichia coli, and is d...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(90)90074-q
更新日期:1990-04-01 00:00:00
abstract::LamB, an integral outer membrane protein of E coli K12, is highly resistant to protease digestion. We had previously genetically inserted a foreign sequence corresponding to an epitope from the poliovirus next to amino acids 146, 153, 189, and 374 of LamB. In 3 cases (sites 146, 153, 374), insertion of the foreign pep...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(90)90144-6
更新日期:1990-02-01 00:00:00
abstract::The repressible Saccharomyces cerevisiae acid phosphatase (APase) coded by the PHO5 gene is a cell wall protein that follows the yeast secretory pathway. We had previously described the in vivo fate of a multicopy plasmid-encoded modified protein, lacking 15 out of 17 signal peptide amino acids. This modified protein ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(90)90135-4
更新日期:1990-02-01 00:00:00
abstract::The published literature contains a number of examples of normally non-cytoplasmic proteins whose transport out of the cytoplasm is not completely abolished by drastic alterations to their routing signals (signal sequences, etc). Furthermore, there are numerous examples of cytoplasmic proteins that can be routed to an...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(90)90133-2
更新日期:1990-02-01 00:00:00
abstract::Total poly (A+) RNA has been extracted from rat pheochromocytoma and translated in vitro by means of a reticulocyte lysate system. We show that two antisera, prepared against pig kidney DOPA decarboxylase (DDC) or rat pheochromocytoma DDC, immunoprecipitate an in vitro synthetized 50 kDa polypeptide identified as DDC ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(90)90175-g
更新日期:1990-01-01 00:00:00
abstract::RNA-protein crosslinks were introduced into the 40S ribosomal subunits from Saccharomyces cerevisiae by mild UV treatment. Proteins crosslinked to the 18S rRNA molecule were separated from free proteins by repeated extraction of the treated subunits and centrifugation in glycerol gradients. After digestion with RNase ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(89)90024-2
更新日期:1989-11-01 00:00:00
abstract::The incubation of polyphosphates with the ribosomal fraction of Sulfolobus acidocaldarius leads to the covalent attachment of phosphate to threonine residue(s) of a single 40,000 Mr protein. The hydrolysis kinetics of this protein showed that polyphosphate might be the modifying group. The reaction requires 2 mM Mn2+ ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(89)90115-6
更新日期:1989-09-01 00:00:00
abstract::The structure of ATP synthase subunit 4 was determined by using the oligonucleotide probe procedure. This subunit is the fourth polypeptide of the complex when classifying subunits in order of decreasing molecular mass. Its relative molecular mass is 25 kDa. The ATP4 gene was isolated and sequenced. The nucleotide seq...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(89)90073-4
更新日期:1989-08-01 00:00:00
abstract::The quantitative formalism called Metabolic Control Theory makes it possible to be precise in discussions of metabolic control. To illustrate this, I will mention 2 experimental systems where free energy is converted from one form to another, i.e., bacteriorhodopsin liposomes and mitochondrial oxidative phosphorylatio...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(89)90071-0
更新日期:1989-08-01 00:00:00
abstract::We have synthesized and examined a number of fully and partly self-complementary palindromic oligodeoxynucleotides for their ability to assume in solution a unimolecular hairpin structure. The main results obtained by a combined optical and electrophoresis investigation show that: (i) DNA folding needs not be driven b...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(89)90042-4
更新日期:1989-07-01 00:00:00
abstract::Unlike most tonoplast ATPases, the vacuolar ATPase of Acer pseudoplatanus cells (Km = 0.4 mM) was strongly inhibited by vanadate (I50 = 10 microM). The inhibition was non-competitive. Chemicals usually added in the reaction mixture either increase (NH+4, K+) or decrease (Na+, EDTA) the ATPase inhibition. However, thes...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(89)90044-8
更新日期:1989-07-01 00:00:00
abstract::The mouse interferon alpha 7 gene, the signal sequence of which has been removed by oligonucleotide-directed mutagenesis, was introduced into a Bacillus subtilis secretion vector containing the promoter and the signal sequence of the B. subtilis levansucrase gene. Different B. subtilis strains were transformed with th...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(89)90091-6
更新日期:1989-06-01 00:00:00
abstract::Hydroxylamine and its derivatives of general formula H2NOR react with aldehydes and aldimines to produce oximes. If R corresponds to the side chain of a natural amino acid, such compounds can be thought of as analogs of the corresponding amino acids, lacking the alpha-carboxylate group. Oximes formed between such comp...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(89)90175-2
更新日期:1989-04-01 00:00:00
abstract::The primary kinetic isotope effect of the reaction catalyzed by NAD+-dependent formate dehydrogenase (EC 1.2.1.2.) from the methylotrophic bacterium Pseudomonas sp. 101 has been studied. Analysis of the ratios HVm/DVm and H(Vm/KM)/D(Vm/KM) in the pH range 6.1-7.9 showed that the transfer of hydride ion in ternary enzy...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(89)90186-7
更新日期:1989-04-01 00:00:00
abstract::The inhibition rates and spectral characteristics of 2 probes specific for the active-site serine residue of proteases were examined for evidence of conformational change of the proteases upon their binding to alpha 2-macroglobulin (alpha 2M). Elastase, chymotrypsin, trypsin, and plasmin were reacted with (7-nitrobenz...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(89)90004-7
更新日期:1989-03-01 00:00:00
abstract::Thyroglobulin is a large-size iodoglycoprotein specific to thyroid tissue and is the substrate for the synthesis of thyroid hormones, thyroxine and 3,5,3'-triiodothyronine. Recent studies, which greatly benefited from recombinant DNA methodologies, improved the knowledge of several structural features of this dimeric ...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(89)90057-6
更新日期:1989-02-01 00:00:00
abstract::The single channel data for 4 different linear gramicidins containing either 4 Trp, 4 Phe, 4 Tyr or TyrBzl have been analyzed on the basis of 3 barriers-2 sites model. They form 2 families which differ by their single channel behavior and thus different energy profiles of the channel. A relationship between the surfac...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(89)90136-3
更新日期:1989-01-01 00:00:00
abstract::The binding of Ca2+ to membrane models composed of diplamitoylphosphatidylcholine (DPPC) and dipalmitoylphosphatidylethanolamine (DPPE) 15N-labeled in the polar head group was investigated at pH 8.5 and pH 9.4 by 15N-NMR spectroscopy. Both phospholipids exhibit a decrease in the chemical shift anisotropy, indicating c...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(89)90143-0
更新日期:1989-01-01 00:00:00
abstract::The effects on intra- and extracellular pH of two polyenic derivatives of amphotericin B, N-fructosyl amphotericin B and N-fructosyl amphotericin B methyl-ester, were tested on HL-60 promyelocytic leukemia cells. Both derivatives raised the internal pH and reduced the external pH in weakly buffered medium. These resul...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(89)90133-8
更新日期:1989-01-01 00:00:00
abstract::Catalase (E.C 1.11.1.6) was purified from leaves of Zandedeschia aethiopica to apparent homogeneity by a one-step hydrophobic interaction chromatography on a phenyl Sepharose CL-4B column. The purified enzyme preparation was obtained with a final recovery of enzyme activity of about 61% and a specific activity of 146 ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(88)90035-1
更新日期:1988-12-01 00:00:00
abstract::At least 6 N-acetylglucosaminyltransferases (GlcNAc-T I, II, III, IV, V and VI) are involved in initiating the synthesis of the various branches found in complex asparagine-linked oligosaccharides (N-glycans), as indicated below: GlcNAc beta 1-6 GlcNAc-T V GlcNAc beta 1-4 GlcNAc-T VI GlcNAc beta 1-2Man alpha 1-6 GlcNA...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(88)90289-1
更新日期:1988-11-01 00:00:00
abstract::Sialate 9(4)-O-acetylesterases (EC 3.1.1.53) have been isolated from equine liver, bovine brain and influenza C virus. In this latter case, the esterase represents the receptor-destroying enzyme of the virus. The kinetic properties of these enzymes were determined with Neu5,9Ac2 and in part with 4-methylumbelliferyl a...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(88)90288-x
更新日期:1988-11-01 00:00:00
abstract::Homeostasis in vivo is maintained by a highly complex network of positive and negative signals. At the cellular level, this regulatory microenvironment can be divided, in a simplified fashion, into two major compartments: the humoral compartment, including compounds such as hormones, growth factors and nutrients, and ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(88)90301-x
更新日期:1988-11-01 00:00:00
abstract::The comparison of the amino acid sequences of 5 cysteine proteinases: papain, actinidin, rat cathepsins B and H and chicken cathepsin L, demonstrates a striking homology among their sequences. The N-terminal region (residues 1-70 in papain) and C-terminal region (residues 118-212 in papain) display the highest sequenc...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(88)90004-1
更新日期:1988-10-01 00:00:00
abstract::Long-term exposure to high doses of caffeine affects several aspects of nitrogen metabolism, such as purine, pyrimidine and urea synthesis. However, little is known about the mechanisms of these changes and if they occur at shorter term. We have studied in isolated hepatocytes: 1) the in vitro effect of high doses of ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(88)90014-4
更新日期:1988-10-01 00:00:00
abstract::Datura stramonium contains a compound that impairs learning retention in mice. It has been purified to homogeneity and its structure has been established as that of a gamma-L-glutamyl-L-aspartate. The biological activity of this pseudodipeptide has been found to be identical with that of the corresponding synthetic on...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(88)90183-6
更新日期:1988-09-01 00:00:00
abstract::Cotranslational translocation of exportable proteins across the RER membrane prior to their release into the extracellular space has been essentially described by use of canine pancreatic microsomal membranes. Intracisternal segregation of nascent secretory proteins was observed to be irreversible and proteolytic remo...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(88)90194-0
更新日期:1988-09-01 00:00:00
abstract::Strains of Escherichia, Salmonella, Shigella and Yersinia actively enter eukaryotic cells. Several techniques were used to compare and contrast the invasion mechanisms of Salmonella cholerae-suis, Yersinia enterocolitica and Shigella flexneri. Three animal cell lines (CHO, HEp-2 and MDCK) were examined for susceptibil...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(88)90271-4
更新日期:1988-08-01 00:00:00
abstract::Cell-free extracts of fungal mycelia of two aflatoxin non-producing isolates of Aspergillus parasiticus (SRRC 163 and SRRC 2043) were utilized for the study of enzyme activities involved in the latter stages of aflatoxin biosynthesis. The post-microsomal fractions (105,000 x g supernatant) of both SRRC 163 and SRRC 20...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(88)90103-4
更新日期:1988-06-01 00:00:00
abstract::We screened a cDNA library of the primitive eukaryote Dictyostelium discoideum constructed in the expression vector lambda gt11 with a specific antiserum directed against S-adenosyl-L-homocysteine hydrolase (AdoHcy hydrolase) and isolated cDNA clones coding for fusion proteins with beta-galactosidase. The identity of ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(88)90115-0
更新日期:1988-06-01 00:00:00
abstract::In vitro cycling rates of E. coli ribosomes and of elongation factors EF-Tu and EF-G have been obtained and these are compatible with translation rates in vivo. We show that the rate of translocation is faster than 50 s-1 and therefore that the EF-G function is not a rate limiting step in protein synthesis. The in viv...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(88)90244-1
更新日期:1988-05-01 00:00:00
abstract::The lactose plasmid pUCL22 of the single plasmid strain Streptococcus lactis ssp. lactis Z270 was demonstrated to fuse with the heterologous conjugative plasmid pVA797. The fusion of pUCL22 with pVA797 occurred by recombination between a specific sequence of pUCL22 and different sites of pVA797. The cointegrates of pU...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(88)90091-0
更新日期:1988-04-01 00:00:00
abstract::Bacteria of the Lactococcus (formerly lactic Streptococcus) genus are described to emphasize certain less well-known facts of importance in their successful use as dairy starters. Other streptococci (non-rod forms) are briefly characterized to emphasize their usefulness in industrial milk fermentations. ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(88)90087-9
更新日期:1988-04-01 00:00:00
abstract::Lactic acid bacteria provide a model system for the in vivo study of mechanisms pertaining to the regulation of sugar transport and metabolism by microorganisms. Recent studies with resting and growing cells of the homofermentative Streptococci and Lactobacilli have yielded evidence for hitherto unsuspected regulatory...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(88)90205-2
更新日期:1988-03-01 00:00:00